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tgf βr2  (Arigo Biolaboratories)

 
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    Arigo Biolaboratories tgf βr2
    Tgf βr2, supplied by Arigo Biolaboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tgf+%CE%B2r2/anti+tgf+%CE%B2/pm41558294-71-60-62
    Average 86 stars, based on 1 article reviews
    tgf βr2 - by Bioz Stars, 2026-09
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    Muscle-specific TGF-β signaling regulates macrophage phenotypes and efferocytosis in inflamed muscle. (A) FACS analysis of the proportion of M1 (F4/80 + Ly6C + ) and M2 (F4/80 + CD206 + ) macrophages. (B) qRT-PCR analysis of gene levels of anti-inflammatory (Arg-1, Mrc1, Retlna) and pro-inflammatory (iNOS, TNF-α, IL-6) molecules in macrophages sorted from damaged muscle of control and <t>SM</t> <t>TGF-βr2</t> -/- mice on day 3 post-myoinjury( n = 3). (C) Immunofluorescence analysis of the efferocytic macrophages (Tunel + F4/80 + ) and the free macrophages (Tunel - F4/80 + ). White arrow indicates the efferocytic macrophages. (D) FACS analysis of the proportion of F4/80 + Tunel + , and F4/80 + Tunel - macrophages. Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm. ns, not significant.
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    Muscle-specific TGF-β signaling regulates macrophage phenotypes and efferocytosis in inflamed muscle. (A) FACS analysis of the proportion of M1 (F4/80 + Ly6C + ) and M2 (F4/80 + CD206 + ) macrophages. (B) qRT-PCR analysis of gene levels of anti-inflammatory (Arg-1, Mrc1, Retlna) and pro-inflammatory (iNOS, TNF-α, IL-6) molecules in macrophages sorted from damaged muscle of control and SM TGF-βr2 -/- mice on day 3 post-myoinjury( n = 3). (C) Immunofluorescence analysis of the efferocytic macrophages (Tunel + F4/80 + ) and the free macrophages (Tunel - F4/80 + ). White arrow indicates the efferocytic macrophages. (D) FACS analysis of the proportion of F4/80 + Tunel + , and F4/80 + Tunel - macrophages. Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm. ns, not significant.

    Journal: Frontiers in Immunology

    Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle

    doi: 10.3389/fimmu.2026.1810106

    Figure Lengend Snippet: Muscle-specific TGF-β signaling regulates macrophage phenotypes and efferocytosis in inflamed muscle. (A) FACS analysis of the proportion of M1 (F4/80 + Ly6C + ) and M2 (F4/80 + CD206 + ) macrophages. (B) qRT-PCR analysis of gene levels of anti-inflammatory (Arg-1, Mrc1, Retlna) and pro-inflammatory (iNOS, TNF-α, IL-6) molecules in macrophages sorted from damaged muscle of control and SM TGF-βr2 -/- mice on day 3 post-myoinjury( n = 3). (C) Immunofluorescence analysis of the efferocytic macrophages (Tunel + F4/80 + ) and the free macrophages (Tunel - F4/80 + ). White arrow indicates the efferocytic macrophages. (D) FACS analysis of the proportion of F4/80 + Tunel + , and F4/80 + Tunel - macrophages. Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm. ns, not significant.

    Article Snippet: Skeletal muscle-specific TGF-β receptor 2 knockout mice (referred to as SM TGF-βr2 −/− ) were generated by crossing MCK-Cre mice (The Jackson Laboratory) with floxed TGF-βr2 mice (TGF-βr2 flox/flox , The Jackson Laboratory).

    Techniques: Quantitative RT-PCR, Control, Immunofluorescence, TUNEL Assay

    Muscle TGF-β signaling prompts to Tregs-mediated macrophage efferocytosis by suppressing myofiber IL-6 production. (A) Western blot and immunofluorescence analysis show IL-6 and p-STAT3 expression in inflamed muscle ( n = 3). (B) Western blot analysis of IL-6 and p-STAT3 protein levels in control or SM TGF-βr2 -/- MPC-myotubes cultured in pro-inflammatory milieu, with or without SRI administration ( n = 3). (C) FACS analysis of the proportion of CD25 + p-STAT3 + cells and CD25 + gp130 + cells in inflamed muscle ( n = 4). (D) FACS analysis of the proportion of CD25 + Foxp3 + cells and CD25 + IL-13 + cells in Tregs co-cultured with TGF-βr2 -/- or control-MPC-myotubes, treated with or without AH or SRI ( n = 3). (E) qRT-PCR analysis of Foxp3 and IL-13 mRNA levels in Tregs co-cultured with TGF-βr2 -/- or control-MPC-myotubes, treated with or without AH or SRI ( n = 3). Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm. ns, not significant.

    Journal: Frontiers in Immunology

    Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle

    doi: 10.3389/fimmu.2026.1810106

    Figure Lengend Snippet: Muscle TGF-β signaling prompts to Tregs-mediated macrophage efferocytosis by suppressing myofiber IL-6 production. (A) Western blot and immunofluorescence analysis show IL-6 and p-STAT3 expression in inflamed muscle ( n = 3). (B) Western blot analysis of IL-6 and p-STAT3 protein levels in control or SM TGF-βr2 -/- MPC-myotubes cultured in pro-inflammatory milieu, with or without SRI administration ( n = 3). (C) FACS analysis of the proportion of CD25 + p-STAT3 + cells and CD25 + gp130 + cells in inflamed muscle ( n = 4). (D) FACS analysis of the proportion of CD25 + Foxp3 + cells and CD25 + IL-13 + cells in Tregs co-cultured with TGF-βr2 -/- or control-MPC-myotubes, treated with or without AH or SRI ( n = 3). (E) qRT-PCR analysis of Foxp3 and IL-13 mRNA levels in Tregs co-cultured with TGF-βr2 -/- or control-MPC-myotubes, treated with or without AH or SRI ( n = 3). Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm. ns, not significant.

    Article Snippet: Skeletal muscle-specific TGF-β receptor 2 knockout mice (referred to as SM TGF-βr2 −/− ) were generated by crossing MCK-Cre mice (The Jackson Laboratory) with floxed TGF-βr2 mice (TGF-βr2 flox/flox , The Jackson Laboratory).

    Techniques: Western Blot, Immunofluorescence, Expressing, Control, Cell Culture, Quantitative RT-PCR

    Myofiber TGF-β signaling regulates Tregs-mediated macrophage efferocytosis in vitro. (A) Scheme of the co-culture experiment. (B) Fluorescence staining shows DiD + ACs engulf by macrophages in co-culture system with or without TGF-βr2 -/- MPC-myotubes, SRI, or AH. (C) FACS analysis of the proportion of F4/80 + CD206 + M2 macrophages and macrophages engulfed DiD + ACs in co-culture system. (D) FACS analysis of the proportion of F4/80 + IL-10 + , F4/80 + p-STAT3 + , F4/80 + Vav1 + and F4/80 + Rac1 + cells. Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm.

    Journal: Frontiers in Immunology

    Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle

    doi: 10.3389/fimmu.2026.1810106

    Figure Lengend Snippet: Myofiber TGF-β signaling regulates Tregs-mediated macrophage efferocytosis in vitro. (A) Scheme of the co-culture experiment. (B) Fluorescence staining shows DiD + ACs engulf by macrophages in co-culture system with or without TGF-βr2 -/- MPC-myotubes, SRI, or AH. (C) FACS analysis of the proportion of F4/80 + CD206 + M2 macrophages and macrophages engulfed DiD + ACs in co-culture system. (D) FACS analysis of the proportion of F4/80 + IL-10 + , F4/80 + p-STAT3 + , F4/80 + Vav1 + and F4/80 + Rac1 + cells. Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm.

    Article Snippet: Skeletal muscle-specific TGF-β receptor 2 knockout mice (referred to as SM TGF-βr2 −/− ) were generated by crossing MCK-Cre mice (The Jackson Laboratory) with floxed TGF-βr2 mice (TGF-βr2 flox/flox , The Jackson Laboratory).

    Techniques: In Vitro, Co-Culture Assay, Fluorescence, Staining